Identification and characterization of Pestalotiopsis spp. causing leaf spot and leaf blight on jabon (Neolamarckia spp.) in Indonesia

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چکیده

Abstract. Herliyana EN, Oktavianto P, Siregar UJ. 2022. Identification and characterization of Pestalotiopsis spp. causing leaf spot blight on jabon (Neolamarckia spp.) in Indonesia. Biodiversitas 23: 6547-6556. Jabon is one the fast-growing trees with high economic value for industrial plantations. Its monoculture planting system faced certain problems, such as diseases caused by sp. Therefore, aim this research was to describe morphology species through macroscopic microscopic observations, followed identification fungus using DNA barcoding tree. A total 11 isolates were used research. The identified observing mycelium colonies fungal conidia, while initiated after isolation cetyltrimethylammonium bromide (CTAB) method, amplification ITS 1 4 primers, sequencing process. results showed that 5 Pestalotiopsis, namely Pseudopestalotiopsis theae, microspora, palmarum, Neopestalotiopsis clavispora, virgatula found associated disease. All five further classified into 3 clades phylogeny according their median cells apical appendage shape. clade X consisted P. N. versicolorous cells, whereas Y included microspora concolorous cell, Z Ps. theae cell knobbed appendage.

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ژورنال

عنوان ژورنال: Biodiversitas

سال: 2023

ISSN: ['1412-033X', '2085-4722']

DOI: https://doi.org/10.13057/biodiv/d231253